Fig. 3: Concomitant with diminished Th2 and ILC2 responses, MIF deficiency leads to impaired eosinophil recruitment and alternative macrophage polarisation in the peritoneal cavity.

Mice of both genotypes were infected with 400 L3 N. brasiliensis larvae by s.c. injection, and samples recovered at day 6 for cellular and ELISA analysis, using the gating strategy described in Fig. S3B. a Total peritoneal lavage cells recovered from infected mice (n = 8) and naive controls (n = 7), combined data from two independent experiments. b Siglec-F⁺ eosinophils recovered from infected mice (n = 5) and naive controls (n = 3) enumerated by flow cytometry. Alternatively activated CD11b⁺F4/80⁺ macrophages evaluated by expression of RELMα (c), and Chil3/Ym1 (d) in samples from infected mice (n = 5) and naive controls (n = 3), enumerated by flow cytometry. e, f Concentrations of RELMα (j) and Chil3/Ym1 (k) in the peritoneal lavage fluid measured by ELISA, in samples from infected mice (n = 3–4) and naive controls (n = 3). Data are presented as arithmetic means and standard errors, and b–f are representative of at least two individually performed experiments; data were statistically analysed by unpaired t tests. *P < 0.05, **P < 0.01.