Fig. 8: Contribution of individual cellular sources of TNF to epithelial cell proliferation and IL-22BP expression in the colon.

A Levels of hTNF and mTNF in ex vivo colonic explants from Rag1^−/− mice following transfer of hTNF-KI T cells into Rag1^−/− mice and treated with Fc control or infliximab for 2 weeks. B Levels of hTNF and mTNF in ex vivo colonic explants from hTNF-KIxRag1^−/− mice that received T cells isolated from WT mice and were treated with Fc control or infliximab for 2 weeks. C hTNF and mTNF levels in sera of colitic Rag1^−/− mice transferred with hTNF-KI naive T cells before anti-TNF therapy. D Analysis of hTNF expression on the surface of blood T cells. Rag1^−/− mice were reconstituted with naive T cells from WT or hTNF-KI mice. Blood cells were analysed once mice had lost 5% of their initial weight. E Expression of IL-22BP in CD4 T cells isolated from WT and hTNF KI spleen. All data are representative of two independent experiments. Data represent mean values ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, as calculated by Student’s t-test; ns not significant.