Fig. 6: Features of mucosal CD4+ T cells triggered by i.n. immunization with LV::S40-HAPEHR or LV::S40-HAPEHR-20. | Mucosal Immunology

Fig. 6: Features of mucosal CD4+ T cells triggered by i.n. immunization with LV::S40-HAPEHR or LV::S40-HAPEHR-20.

From: A lentiviral vector expressing a dendritic cell-targeting multimer induces mucosal anti-mycobacterial CD4+ T-cell immunity

Fig. 6

C57BL/6 mice (n = 3/group) were immunized i.n. with 1 × 108 TU of LV::S40-HAPEHR or LV::S40-HAPEHR-20. On 14 dpi, lung CD4+ T cells were distinguished by their location within the interstitium (CD45i.v−) or vasculature (CD45i.v+) by an i.v. injection of PE-anti-CD45 mAb. a Profile of CD27 vs CD62L or CD45RB, and b CD103 vs CD69, or CD44 vs CXCR3 of lung CD4+ T cells of the interstitium or vasculature. c Percentage of (poly)functional CD4+ T cells specific to EsxA, PE19, or EspC in the lung interstitium or vasculature, as determined by ICS. Results, representative of two independent experiments, were obtained using pooled lungs for each group (n = 3/group). Individual points represent technical duplicates. Results are representative of at least three independent experiments.

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