Fig. 3: IgEG and IgEE clonotypes.
From: B-cell receptor physical properties affect relative IgG1 and IgE responses in mouse egg allergy
Sequences from allergic mice were analyzed after 13 o.g. allergen challenges. a Shared clonotypes (VH-genes) were ordered in accordance to the ratios of their IgE to IgG1 copy numbers. Representative data from one mLN and one BM sample are shown. b Shared clonotypes from the 50 most abundant clonotypes. c Shared clones were classified as IgE-biased (IgEE, IgE/IgG copy number ratio of ≥ 2), or IgG1-biased (IgEG, IgG/IgE copy number ratio of ≥ 2). Copy numbers of mutated IgEE (mIgEE), mutated IgEG (mIgEG), unmutated IgEE (umIgEE), and unmutated IgEG (umIgEG) from BM and mLN are shown, as indicated. Each symbol represents the mean copy numbers form one mouse for the IgE subtype indicated. mLN samples from 5 allergic mice from one experiment and BM samples from 7 mice pooled from two independent experiments were analyzed (mLN: n = 5, BM: n = 7). No sample was excluded from the analysis. d Hypermutation rates of IgEG and IgEE. Mean percentage of non-silent mutations within the IgH-CDR3 regions of mutated IgEE (muIgEE), mutated IgEG (muIgEG), unmutated IgEE (umIgEE) and unmutated IgEG (umIgEG) are shown for BM and mLN, as indicated. Data presented as mean ± SEM. Statistics: pairwise t-test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
