Fig. 4: Physiochemical properties of IgEG and IgEE.
From: B-cell receptor physical properties affect relative IgG1 and IgE responses in mouse egg allergy
Sequences from mLN and BM of allergic mice were analyzed after 13 o.g. allergen challenges. Physiochemical properties of IgEE and IgEG clones were calculated using the “Peptide” package in the R program. Aliphatic index, hydrophobicity index according to Kyte-Doolittle, net charges and isoelectric point (PI), are shown for mutated IgEE (muIgEE), mutated IgEG (mIgEG), unmutated IgEE (umIgEE) and unmutated IgEG (umIgEG). mLN samples from 5 allergic mice from one experiment and BM samples from 7 mice pooled from two independent experiments were analyzed. Two BM samples did not contain detectable umIgEE/ umIgEG. a Data from BM (n = 5–7). b Data from mLN (n = 5). Each symbol represents the respective average ratios form one mouse. Data presented as mean ± SEM. Statistics: pairwise t-test. *p ≤ 0.05; **p ≤ 0.01.
