Fig. 8: Clonal distribution of somatic hypermutation rates and lineage tree analysis.
From: B-cell receptor physical properties affect relative IgG1 and IgE responses in mouse egg allergy
cDNA from allergic mice challenged o.g. 13 times with EG/EYP was analyzed by NGS. Individual clonotypes within the IgG1 and IgE IgH-repertoires were defined by unique VDJ-rearrangements. a Distribution of somatic hypermutation (SHM) rates of shared IgE and IgG1 clonotypes in one representative samples. b Statistical analysis of the distribution of somatic hypermutation of shared clonotypes in samples from BM of a total of 7 mice from two independent experiments (n = 7). Number of shared IgE clones with various hypermutation rates are shown, as indicated. c Analysis of lineages trees of highly mutated BM IgE clonotypes. Three samples containing less than 10 highly mutated IgE clonotypes were excluded from the analysis (n = 4). The number of highly mutated IgE (≥ 12 mutations) clonally related to highly mutated IgG1 (≥ 12 mutations) and low/moderately mutated IgG1 (≤ 11 mutations) are shown. (b, c) Each symbol represents data from one mouse and colored bars represent the mean. In total, lineage trees of 40 highly mutated IgE clonotypes were analyzed. d–h Examples of lineage trees of highly mutated IgE clonotypes are shown. Data presented as mean ± SEM. Statistics: Paired parametric t-test *p ≤ 0.05; **p ≤ 0.01.
