Fig. 2: SpikeAx IT vaccinated mice retain lung-resident memory T cells up to eight weeks post-vaccination. | Mucosal Immunology

Fig. 2: SpikeAx IT vaccinated mice retain lung-resident memory T cells up to eight weeks post-vaccination.

From: Mucosal immunization with a delta-inulin adjuvanted recombinant spike vaccine elicits lung-resident immune memory and protects mice against SARS-CoV-2

Fig. 2

C57BL/6 mice were vaccinated as in Fig. 1 and at eight weeks post-boost mice lungs were collected for flow cytometric analysis. A Percentage of total CD4+ T cells in the lungs expressing TRM-like phenotype (CD44+CD69+CD62L−). B Percentage of total CD4+ T cells in the lungs expressing RorγT. C Percentage of total CD8+ T cells in the lungs expressing TRM-like markers (CD44+CD69+CD103+CD62L−). D, E Representative FACS plots of lung CD4+ T cells. F Representative FACS plots of lung CD8+ T cells. Bar graphs (A–C) depict individual and mean values +/− SEM of five mice per group and are representative of more than two independent experiments. Statistical analysis of differences between groups was performed using a one-way ANOVA with Tukey’s multiple comparisons test, p < 0.05 (*), p < 0.005 (**), p < 0.0005 (***). FACS plots (D–F) are representative of the mice shown in graphs (A–C).

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