Fig. 4: SpikeAx IT induces lasting lymphocytic recruitment to the airways. | Mucosal Immunology

Fig. 4: SpikeAx IT induces lasting lymphocytic recruitment to the airways.

From: Mucosal immunization with a delta-inulin adjuvanted recombinant spike vaccine elicits lung-resident immune memory and protects mice against SARS-CoV-2

Fig. 4

C57BL/6 mice were vaccinated as in Fig. 1 and at eight weeks after the final vaccination the bronchoalveolar lavage fluid (BALF) was collected for antibody and flow cytometric analysis; intravascular staining was performed to exclude circulating cells. A Total BALF cell number. B–E Total number of CD4+ T cells, CD8+ T cells, B cells, and alveolar macrophages in the BALF. F–G Percentage of CD4+ T cells in the BALF expressing RorγT. Bar graphs show individual values of four mice per group +/− SEM and are representative of more than two independent experiments. FACS plots are representative of more than two independent experiments. The significance between groups was determined via a one-way ANOVA with Tukey’s multiple comparisons test, p < 0.05 (*), p < 0.005 (**), p < 0.0005 (***).

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