Fig. 1
Generation of Nptx2 conditional knockout mice. a Nptx2 floxed mice were crossed with Sox1::Cre mice to knockout Nptx2 specifically in the brain from early development or with CAG::CreERT2 to knockout Nptx2 in adulthood. We confirmed the high knockout efficiency of Nptx2 in the hippocampus at mRNA (n = 8) and protein levels (n = 6) for both models. b Immunohistochemistry confirmed the absence of NPTX2-positive cells in both the hippocampus and amygdala. c NPTX2 co-localized with NeuN (a marker for neurons), but not GFAP (a marker for glia). Gray arrowheads indicate no colocalization. White arrowheads indicate colocalization of two markers. Scale bar represents 200 µm. Values represent mean ± SEM. ***p < 0.001
