Fig. 1: Breeding scheme used to generate F1, F2, and F3 offspring with control and poly(I:C)-derived ancestors.
From: Transgenerational modification of dopaminergic dysfunctions induced by maternal immune activation
a Breeding scheme used to generate F1 and F2 generations with control and immune-challenged ancestors. Pregnant F0 mice were treated with vehicle control (CON) or poly(I:C) (POL) solution to obtain F1 CON and F1 POL offspring. To obtain F2 POL offspring via the paternal lineage (POL-PL), male F1 POL offspring were mated with female F1 CON offspring; and to generate F2 POL offspring via the maternal lineage (ML-POL), female F1 POL offspring were crossed with male F1 CON offspring. F1 CON males and females were crossed to obtain the F2 control lineage (F2 CON). b Breeding scheme used to obtain the F3 generation with control and immune-challenged ancestors deriving from the paternal lineage (PL). Pregnant F0 mice were treated with control (CON) or poly(I:C) (POL) solution, and F1 POL males were mated with F1 CON females to generate F2 POL-PL offspring. F2 POL-PL males were then mated with F2 CON females to obtain F3 POL-PL offspring. CON F3 offspring were generated by crossing F2 CON males and females.
