Fig. 2: Effects of maternal immune activation on the locomotor reaction to acute systemic amphetamine challenge in F1, F2, and F3 offspring.

All animals were first placed into the open field and allowed to habituate for 30 min, after which they were injected with amphetamine (Amph; 2.5 mg/kg) or saline (Sal) solution. The line plots show the distance moved as a function of 5-min-bins during the initial habituation and subsequent drug phase; the bar plots depict the mean distance moved during the drug phase. a Locomotor activity levels in F1 offspring of control (CON) and poly(I:C)-treated (POL) mothers. The data represent male and female offspring together, as there were no sex-specific effects of MIA on Amph sensitivity in F1 offspring. b Locomotor activity levels in male F2 CON offspring and male F2 POL offspring generated via the maternal (POL-ML) or paternal (POL-PL) lineages. c Locomotor activity levels in male F3 CON offspring and male F3 POL offspring generated via the paternal lineage (POL-PL). *p < 0.05, **p < 0.01, and ***p < 0.001, based on post-hoc comparisons. All values are means + SEM. The numbers of animals in each group are provided in Table 1.