Fig. 4: ER272 induces proliferation via classical PKC activation in NPC cultures. | Neuropsychopharmacology

Fig. 4: ER272 induces proliferation via classical PKC activation in NPC cultures.

From: Effects of classical PKC activation on hippocampal neurogenesis and cognitive performance: mechanism of action

Fig. 4

A Representative phase-contrast microscopy images of neurospheres cultured for 72 h in presence or absence of the basic fibroblastic growth factor (bFGF), ER272 or the classical PKC inhibitor (Gö6976). Scale bar indicates 100 µm. B Graph shows the effect of the different treatments on neurospheres area expressed as the percentage of control (bFGF treatment). Data shown are the mean ± S.E.M. of nine independent measurements. Statistical analysis: *p = 0.037 in two tailed unpaired Student’s t test comparing bFGF with bFGF+ER272. C Flow cytometry analysis of cells isolated from neurospheres treated under the different conditions indicated and labelled with anti- Ki67 antibody; left panel: forward/side scatter; right panel: cell count vs. fluorescence intensity in the six different conditions indicated. D Quantification of proliferating cells (Ki67+) in flow cytometry plots as those shown in (C). Statistical analysis: one-way ANOVA test: ***p = 0.0005, bFGF + ER272 vs. bFGF; ***p = 0.0004 bFGF + ER272 vs. bFGF + siRNA α; ***p < 0.0001 bFGF + ER272 vs. bFGF + ER272 + siRNA α; *p = 0.0165, bFGF + ER272 vs. bFGF + ER272 + siRNA β.

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