Fig. 2: EphB2+/− females, but not males, display repetitive behaviors and hyperactivity.
From: Sex-dependent role for EPHB2 in brain development and autism-associated behavior
A Both WT and EphB2+/− male and female mice show a significant preference for a novel mouse over a novel object in a 3-arena social interaction assay, with no genotype differences (three-way ANOVA; main effect of IZ: p = 0.0002). B EphB2+/− male and female pups show no difference in the number of USVs at P5/6 or P10 compared to their WT littermates during maternal separation (three-way ANOVA). EphB2+/− male and female mice show normal anxiety-like behavior (two-way ANOVA) in the elevated plus maze (C) and open field (D). E EphB2+/− mice show an increase in repetitive rearing (two-way ANOVA; main effect of genotype; p = 0.0234), post hoc analysis shows a statistical trend to an increase in EphB2+/− females, but not in males (post hoc analysis: for females, p = 0.054, and for males, p = 0.4986). F EphB2+/− females, but not males, show repetitive horizontal movements (two-way ANOVA; no main effect of genotype but significant interaction: p = 0.0251, post hoc analysis: for females, p = 0.0385, and for males, p = 0.6315). G No difference in repetitive jumping between EphB2+/− and WT mice (two-way ANOVA). H EphB2+/− mice are hyperactive (two-way ANOVA; main effect of genotype: p = 0.0011), post hoc analysis shows a significant hyperactivity in EphB2+/− females, but not in males (post hoc analysis: for females, p = 0.0018, and for males, p = 0.3034). WT wild type, IZ interaction zone, USVs ultrasonic vocalizations. Data are represented as mean ± SEM. Statistical significance was determined by two-way ANOVA or three-way ANOVA. #p < 0.1, *p < 0.05, **p < 0.005, ***p < 0.0005, ns not significant. The number of animals is indicated within each bar for each experiment.
