Fig. 4: TRAP analysis of PV interneuron after chronic treatment with fluoxetine. | Neuropsychopharmacology

Fig. 4: TRAP analysis of PV interneuron after chronic treatment with fluoxetine.

From: Activation of TrkB in Parvalbumin interneurons is required for the promotion of reversal learning in spatial and fear memory by antidepressants

Fig. 4

a Ribosome-tagged transgenic mice were treated with fluoxetine or control water for 2 weeks, and their hippocampi were isolated and lysated. Ribosomes bound to mRNA were immunoprecipitated with beads coated with GFP-antibody and the mRNA was purified for cDNA synthesis followed by next-generation sequencing (NGS). b Immunohistochemistry analysis with anti-PV antibody. Parvalbumin is co-localized with GFP indicating that the cells expressing GFP-tag in ribosomes are PV cells. Scale bars, 50 µm. c Volcano plot showing log2 of fold change of all genes after fluoxetine treatment in x-axis and negative log10 of P value in y-axis. Downregulated genes that had significantly differential expression are marked in blue and upregulated in red. d Heatmap of significant genes and pathways detected by GO analysis. The expression of genes in a sample is scaled to values between −2 and 2, and these correlate with colors in the heatmap according to the panel on the right. GAGs C glycosaminoglycan biosynthesis chondroitin sulfate, GAGs H glycosaminoglycan biosynthesis heparan sulfate, GPL glycerophospholipid metabolism, GL glycerolipid metabolism.

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