Fig. 5: Ketamine affects synaptic tuning at multiple steps.
From: The effect of ketamine on synaptic mistuning induced by impaired glutamate reuptake
A Time course of normalized fEPSP slope while stimulating at the indicated frequencies; where indicated, DL-TBOA and either ketamine or BDNF were applied (n = 6 slices each). B Average fEPSP slope measured 15 min after ketamine or BDNF (see A) versus DL-TBOA alone (from Fig. 4B-a). *p < 0.05 versus DL-TBOA alone (ANOVA). C Protocol used to test the effect of pretreating slices with 20 µM ketamine on synaptic plasticity in hippocampal slices from SD rats. D Time course of fEPSP slope normalized to baseline before LTP induction; where indicated, a subthreshold θ-burst stimulation was applied (n = 4–5 slices each). Representative traces shown on top. E Time course of normalized fEPSP slope measured in slices from SD and FSL rats. Where indicated, ketamine was applied to the FSL slices (n = 10–13 slices each). Representative traces shown on top. F Summary of normalized fEPSP slope measured 1 h after LTP induction under the indicated conditions *p < 0.05 versus DL-TBOA and §p < 0.05 versus the corresponding aCSF group (Student’s t-test). G Cartoon illustrating that homeostatic types of plasticity and activity induced potentiation act together to tune synaptic transmission, and that ketamine can act at several mechanisms to stabilize the tuning.
