Fig. 3: DR expression of c-Fos in MOR-VGaT neurons in hyperalgesia during spontaneous heroin withdrawal.
From: Inhibition of dorsal raphe GABAergic neurons blocks hyperalgesia during heroin withdrawal
A Proportion of subpopulations of DR neurons expressing c-Fos. B DR confocal images of the subpopulation of neurons expressing c-Fos at a magnification of 20X, showing detection of c-Fos protein (white), VGaT mRNA (green), MOR mRNA (red), or TPH protein (cyan). C Heroin-treated mice exhibited a higher number of c-Fos-MOR-VGaT neurons than saline-treated mice (**p = 0.0048). D DR regional distribution of c-Fos-MOR-VGAT neurons in heroin treated mice showing higher concentration in the DR lateral subregion (DRL) than in the DR dorsomedial (DRD; *p = 0.0155), DR ventromedial (DRV; ***p = 0.0005), DR interfascicular (DRI; ***p = 0.0008), or DR posterodorsal (PDR; ****p = 0.0004) subregions. E DR rostrocaudal distribution of c-Fos-MOR-VGAT neurons. F DR schematic representation showing concentration of c-Fos-MOR-VGAT neurons in the DRL subregion at bregma -4.48. The data in (A, C, D and E) are expressed as mean ± SEM. (n = 3 mice/group). Aq aqueduct. Scale bars: 5 µm (B).
