Fig. 4: DR expression of c-Fos in MOR-VGluT3 and MOR-VGluT3-TPH neurons in hyperalgesia during spontaneous heroin withdrawal.
From: Inhibition of dorsal raphe GABAergic neurons blocks hyperalgesia during heroin withdrawal
A Proportion of subpopulations of DR neurons expressing c-Fos. B DR confocal images of the subpopulation of neurons expressing c-Fos at a magnification of ×20, showing detection of c-Fos protein (white), VGluT3 mRNA (green), MOR mRNA (red), or TPH protein (cyan). C Heroin-treated mice exhibited a higher number of c-Fos-MOR-VGluT3 neurons than saline-treated mice (**p = 0.0027). D Heroin-treated mice exhibited a higher number of c-Fos-MOR-VGluT3-TPH neurons than saline-treated mice (**p < 0.0001). The data in (A, C and D) are expressed as mean ± SEM. (n = 3 mice/group). Scale bars: 5 µm (B).
