Fig. 5: Inhibition of DR-VGaT neurons blocks hyperalgesia during heroin withdrawal.
From: Inhibition of dorsal raphe GABAergic neurons blocks hyperalgesia during heroin withdrawal
A DR viral injections of AAV2-hSyn-DIO-hM4Di-mCherry or AAV2-hSyn-DIO-mCherry in VGaT::Cre mice (n = 37). B Representative image (magnification of ×5) of the expression of AAV2-DIO-hSyn-hM4D (Gi)-mCherry in DR-VGaT neurons at bregma -4.36 mm from a heroin-treated VGaT::Cre mouse. C Heroin-treated mCherry control mice exhibited lower paw withdrawal thresholds (hyperalgesia) than saline-treated hM4Di-mCherry (####p < 0.0001) and mCherry control mice (#### p < 0.0001). I.p. injections of J60 increased thresholds in heroin-treated hM4Di-mCherry mice compared with vehicle (****p < 0.0001) and with heroin-treated mCherry control mice treated with either vehicle (****p < 0.0001) or J60 (****p < 0.0001). n = 16-21/group. gf, grams of force. The data in (C) are expressed as mean ± SEM. Scale bars: 100 µm (B).
