Fig. 3: NK3R signaling mildly affects aggression in IST mice.

Schematic drawings depicting the detailed protocols followed in experiments (Exp) C. Please note that each individual Exp has used different cohorts of animals. Osan: Osanetant (5 μg/Kg); RI: resident-intruder; Senkt: senktide (NK3R agonist), 1 μg/kg WO: Washout (neither behavior nor injection) (A). Senktide (light orange bars) reduced the total percentage of time spent on aggression (two-tailed Student’s t test t(11) = 2.96, p = 0.01) and increased percentage of time spent exploring the homecage (t(11) = 3.1, p = 0.01) but it did not affect the number of attacks, attack latency, and social investigation in IST mice (B). Osanetant (dark orange bars) did not affect the total percentage of time spent on aggression, the number of attacks, the latency to attack, or social investigation or homecage exploratory behaviors (C). *p < 0.05 vs vehicle. Data are presented as mean + s.e.m.