Fig. 2: Effects of ICV PACAP38 administration on swim stress-induced c-Fos expression in selected brain regions. | Neuropsychopharmacology

Fig. 2: Effects of ICV PACAP38 administration on swim stress-induced c-Fos expression in selected brain regions.

From: PACAP regulates neuroendocrine and behavioral stress responses via CRF-containing neurons of the rat hypothalamic paraventricular nucleus

Fig. 2: Effects of ICV PACAP38 administration on swim stress-induced c-Fos expression in selected brain regions.

A Schematic coronal sections from the brain atlas [54] showing brain regions examined (gray shaded). B Representative photomicrographs showing c-Fos positive cells in rats exposed to forced swim stress and microinjected either with aCSF or PACAP38. The lower panels show c-Fos quantification presented as bar graphs in the PVN (C) LS (D) and BNST (E). There was a significant increase of stress-induced c-Fos expression in the parvocellular part of the PVN, ventral LS and latero-dorsal part of the BNST of PACAP38-treated animals compared to aCSF-injected controls. Abbreviations: 3 V third ventricle, AC anterior commissure, BNST bed nucleus of the stria terminalis, BNSTld latero-dorsal part of the BNST, BNSTlp latero-posterior part of the BNST, BNSTma medial-anterior part of the BNST, CC corpus callosum, LS lateral septum, LSd lateral septum dorsal, LSv lateral septum ventral, LV lateral ventricle, opt optic nerve, PVN paraventricular nucleus of the hypothalamus, pcPVN parvocellular part of the PVN, mcPVN magnocellular part of the PVN. N = 6–8 animals per group. Scale bars: 100 µm at lower magnification, 25 µm at higher magnification images. Data are expressed as mean ± SEM. *p < 0.05 vs aCSF-injected controls (Student’s t-test).

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