Fig. 5: Distribution of PAC1 receptors in the PVN. | Neuropsychopharmacology

Fig. 5: Distribution of PAC1 receptors in the PVN.

From: PACAP regulates neuroendocrine and behavioral stress responses via CRF-containing neurons of the rat hypothalamic paraventricular nucleus

Fig. 5: Distribution of PAC1 receptors in the PVN.

Representative immunofluorescence photomicrograph from a rat exposed to forced swim stress showing PAC1 (green), c-Fos (blue) and CRF immunolabeling (red) in the PVN. A PAC1 receptors are widely distributed in different subareas of the PVN including the magnocellular (mcPVN) and parvocellular part (pcPVN). Moreover, PAC1 receptor immunoreactivity was found on stress-activated (c-Fos positive) neurons in both the magnocellular and parvocellular part. Notably, within the medial parvocellular part of the PVN (mpcPVN) a considerable portion of PAC1 positive neurons are also labelled for c-Fos and CRF (triple-immunofluorescence staining) indicative for a functional role of PAC1 receptors within this area. B shows representative triple-labelled neurons within the mpcPVN at higher magnification. C The insert in (B) in higher magnification (3D reconstructed deconvoluted imaged) demonstrating two CRF-containing neurons which are c-Fos positive (blue nuclei) and the CRF-expressing neurons express the PAC1 receptor in their membrane (green) as indicated by arrows. Scale bars: (A), 100 µm; (B), 25 µm; (C), 10 µm.

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