Fig. 4: MA-induced potentiation of DR 5-HT neurons is dependent on non-functional TAAR1.

Control refers to the firing frequency, activity, and RMP in the presence of antagonists for 5HT1A (WAY 100635, 100 nM) and 5HT1B (SB 216641, 200 nM) autoreceptors. A Mean firing frequency of MAHDR- Taar1m1J/m1J DR 5-HT neurons pre- and post-MA application [7 recordings (7 mice: Male=4, Female=3); Tukey’s HSD post hoc test, ***p < 0.001 for control compared to MA at a given current. Means ± SEM are presented collapsed on sex.] B Representative traces of MAHDR-Taar1m1J/m1J DR 5-HT recordings at 0 pA and +40 pA current injections in the absence (black) and presence of MA (teal). C RMPs of MAHDR-Taar1m1J/m1J DR 5-HT neurons were significantly depolarized by MA, *p < 0.05. Each set of symbols represents a recording in the absence and presence of MA. D Mean firing frequency of MAHDR-Taar1+/+ KI DR 5-HT neurons pre- and post-MA application [6 recordings (6 mice: Male=3, Female = 3). Means ± SEM are presented collapsed on sex] E Representative traces of MAHDR-Taar1+/+ KI DR 5-HT recordings at 0 pA and +40 pA current injections in the absence (black) and presence of MA (teal). F RMPs of MAHDR-Taar1+/+ KI DR 5-HT neurons were unchanged by MA. Each set of symbols represents a recording in the absence and presence of MA.