Fig. 2: The 4-hydroxy-tamoxifen (4-TM) dependency of tagging with hM4Di-mCherry and silencing (prelimbic) PL ensembles tagged with hM4di-mCherry disrupts cue-evoked sucrose seeking. | Neuropsychopharmacology

Fig. 2: The 4-hydroxy-tamoxifen (4-TM) dependency of tagging with hM4Di-mCherry and silencing (prelimbic) PL ensembles tagged with hM4di-mCherry disrupts cue-evoked sucrose seeking.

From: Prelimbic cortical excitatory overdrive and inhibitory underdrive accompany environmental suppression of food seeking

Fig. 2

a Representative PL images and b levels of hM4Di-mCherry expression from mice not injected with 4-TM in home cage (4-TM−/HC; n = 8); injected with 4-TM in home cage (4-TM+/HC; n = 8) and following a conditioning session (4-TM+/S); n = 8). c Experimental timeline for tagging PL ensembles with hM4Di-mCherry during cue-evoked sucrose seeking and ensemble silencing on test day. d Representative images of PL tagged with hM4Di-mCherry following sucrose cue and novelty exposure. e Disruption of cue-evoked sucrose seeking following clozapine in FosTRAP2 mice tagged with hM4Di-mCherry following sucrose cue exposure (Sucrose FosTRAP2, n = 8); but not following novelty (Novel FosTRAP2, n = 8); and WT control mice that underwent sucrose cue exposure (Sucrose WT, n = 7). *p < 0.05, **p < 0.01 and ****p < 0.0001. All data are expressed as mean ± SEM.

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