Fig. 3: Actigraphy-based quantification of sleep and wake states.
From: Inflammatory pain in mice induces light cycle-dependent effects on sleep architecture
A Mice were individually housed in cages on automated piezoelectric sleep monitoring systems. Sleep and wake behavior were recorded for 2 days of baseline, 7 days after saline injection, and 7 days after CFA injection. Data presented here is for the first 24 h after each injection. As percent of average baseline (dotted line at 100%), B sleep duration (between-groups: t15 = 1.629, p = 0.1242; within-group SAL: t15 = 5.192, p < 0.0001; within-group CFA: t15 = 2.592, p = 0.0204), C sleep bout length (between-groups Wilcoxon: p = 0.0013; within-group SAL: p = 0.3225; within-group CFA: p = 0.0027), and D wake bout length (between-groups Wilcoxon: p = 0.0042; within-group SAL: p = 0.0155; within-group CFA: p < 0.0001) during lights-on. As percent of average baseline E sleep duration (between-groups Wilcoxon: p = 0.0027; within-group SAL: p < 0.0004; within-group CFA: p < 0.0001), F sleep bout length (between-groups: t15 = 0.1013, p = 0.9207; within-group SAL: t15 = 4.1071, p = 0.0010; within-group CFA: t15 = 3.652, p = 0.0024), and G wake bout length (between-groups: t15 = 5.745, p < 0.0001; within-group SAL: t15 = 4.485, p = 0.004; within-group CFA: t15 = 15.34, p < 0.0001) during lights-off. Teal squares indicate males and magenta circles indicate females. Ns indicates not significant. #indicates significant difference from theoretical baseline of 100% (#p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001). *indicate significant group differences (*p < 0.05, **p < 0.01, ***p < 0.001).
