Fig. 4: EEG- and EMG-based quantification of sleep telemetry after CFA injection during lights-on.
From: Inflammatory pain in mice induces light cycle-dependent effects on sleep architecture
A Mice were implanted with wireless telemetry transmitters. EEG and EMG were recorded for 7 days of baseline, 7 days after isoflurane exposure, 7 days after saline injection, and 21 days after CFA injection. Data presented here is for the first 24 h after each injection. Mice were injected at the beginning of the light phase. As percent of average baseline (dotted line at 100%), B REM duration (F(1.986,19.86) = 0.02847, p = 0.9714), C REM bout number (F(1.427,14.27) = 0.745, p = 0.4489), D SWS duration (F(1.390,13.90) = 4.341, p = 0.0457; secondary analysis of sexes individually: Time x Sex interaction: F(2,18) = 6.197, p = 0.0090; Female ISO vs. CFA: p = 0.0133; SAL vs. CFA: p = 0.0302), and E SWS bout number (F(1.960,19.60) = 0.8844, p = 0.4269) during lights-on. F Summary radar plot indicating direction of change between saline and CFA for lights-on. As percent of average baseline, G REM duration (F(1.446,13.01) = 10.72, p = 0.0032, ISO vs. CFA p = 0.0224, SAL vs. CFA p = 0.0115; within-group CFA: t9 = 3.462, p = 0.0071), H REM bout number (F(1.716,17.16) = 6.088, p = 0.0126, SAL vs. CFA p = 0.0092; within-group ISO: t10 = 2.740, p = 0.0208; within-group CFA: t10 = 3.455, p = 0.0062; secondary analysis of sexes individually: main effect of Time: F(1.489,13.40) = 6.893, p = 0.0130; Male SAL vs. CFA p = 0.0443), I SWS duration (F(1.976,28.66) = 8.823, p = 0.0011, ISO vs. CFA p = 0.0112, SAL vs. CFA p = 0.0170; within-group SAL: t10 = 3.188, p = 0.0097; within-group CFA: t10 = 6.548, p < 0.0001), and J SWS bout number (Friedman test F = 6.545, p = 0.0435, SAL vs. CFA p = 0.0315; within-group Wilcoxon CFA: p = 0.0010) during lights-off. K Summary radar plot indicating direction of change between saline and CFA for lights-off. Teal squares indicate males and magenta circles indicate females. Ns indicates not significant. #indicates significant difference from theoretical baseline of 100% (#p < 0.05, ##p < 0.01, ####p < 0.0001). *indicate significant group differences (*p < 0.05, **p < 0.01).
