Fig. 5: EEG- and EMG-based quantification of sleep telemetry after CFA injection during lights-off.
From: Inflammatory pain in mice induces light cycle-dependent effects on sleep architecture
A Mice were implanted with wireless telemetry transmitters. EEG and EMG were recorded for 7 days of baseline, 7 days after isoflurane exposure, 7 days after saline injection, and 21 days after CFA injection. Data presented here is for the first 24 h after each injection. Mice were injected at the beginning of the dark phase. As percent of average baseline (dotted line at 100%), B REM duration (F(1.736,13.89) = 6.291, p = 0.0136, ISO vs. CFA p = 0.0313; within-group CFA: t8 = 3.466, p = 0.0085; secondary analysis of sexes individually: main effect of Time: F(1.558,10.91) = 8.791, p = 0.0075, main effect of Sex: F(1,7) = 6.058, p = 0.0434; Female ISO vs. CFA, p = 0.0245), C REM bout number (F(1.671,13.37) = 2.908, p = 0.0961; within-group ISO t8 = 2.429, p = 0.0413; within-group CFA t8 = 2.794, p = 0.0234), D SWS duration (F(1.571,12.57) = 15.37, p = 0.0007, ISO vs. CFA p = 0.0002, SAL vs. CFA p = 0.0127; within-group CFA: t8 = 9.234, p < 0.0001; secondary analysis of sexes individually: (Time F(1.402,9.816) = 13.83, p = 0.0025; Female ISO vs. CFA p = 0.0020), and E SWS bout (F(1.683,13.47) = 5.901, p = 0.0177, ISO vs. CFA p = 0.0305, SAL vs. CFA p = 0.0225; within-group CFA: t8 = 7.063, p = 0.0001) number during lights-off. F Summary radar plot indicating direction of change between saline and CFA for lights-off. As percent of average baseline, G REM duration (F(1.240,9.919) = 1.053, p = 0.3478), H REM bout number (F(1.826,14.61) = 0.259, p = 0.7555), I SWS duration (F(1.948,15.58) = 8.956, p = 0.0027, ISO vs. CFA p = 0.0062; within-group CFA: t8 = 4.117, p = 0.0034; main effect of Time F(1.928,13.50) = 8.119, p = 0.0051; Female ISO vs. CFA p = 0.0149), and J SWS bout number (F(1.619, 12.96) = 6.095, p = 0.0175, SAL vs. CFA p = 0.0193) during lights-on. K Summary radar plot indicating direction of change between saline and CFA for lights-on. Teal squares indicate males and magenta circles indicate females. Ns indicates not significant. #indicates significant difference from theoretical baseline of 100% (#p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001). *indicate significant group differences (*p < 0.05, **p < 0.01, ***p < 0.001).
