Fig. 4: CBD effects on voluntary alcohol vapor and saccharin self-administration.

A Time course of alcohol vapor self-administration for CBD (green) and vehicle (yellow); two-way repeated measures ANOVA: significant time × treatment interaction (F(25,725) = 2.421, p = 0.0001). B Average vapor exposure (2-min, 5-min, 10-min phases); two-way ANOVA: significant exposure time × treatment interaction (F(2,58) = 7.989, p = 0.0009), Holm-Sidak post-hoc: CBD reduced 10-min phase (p = 0.0041 vs. vehicle), no effect at 2-min or 5-min. C Average responses in the alcohol-paired and inactive nose pokes for vehicle and CBD treated animals during the last 3 days of the 10 min phase; two-way ANOVA: significant nose-poke × treatment interaction (F(1,29) = 12.78, p = 0.0013), Holm-Sidak post-hoc: CBD reduced nose pokes only in the alcohol-paired side (p = 0.0001 vs. vehicle). D Time course of saccharin self-administration for CBD (green) and vehicle (light blue); two-way repeated measures ANOVA: no treatment effect (F(1,18) = 0.9202, p = 0.3501) or time × treatment interaction (F(25,450) = 0.8611, p = 0.6609). E Average responses in the saccharin and water paired levers for vehicle and CBD treated animals during the last 3 days of the study. No lever x treatment interaction (F(1,18) = 0.087, p = 0.77), significant main effect of lever (F(1,18) = 25.51, p = 0.0001). Individual data points for male (blue circles) and female (pink circles) rats are shown. Data as mean ± SEM. **p < 0.01.