Fig. 1: MDD-associated m6A RNA methylation profiling and annotation in dorsolateral prefrontal cortex following microarray.

A An expression heatmap showing normalized methylation enrichment values of RNA transcripts was determined across samples based on a group-wise comparison between control subjects (n = 49) and MDD subjects (n = 49). A cluster dendrogram has been added by clustering the 100 differentially methylated transcripts across the samples based on their hierarchical clustering following similarities in their expression pattern. B Volcano plot from microarray m6A methylation data analysis. Red and blue dots indicate hypomethylated and hypermethylated RNA transcripts from microarray methylation data analysis results. C Circular plot showing chromosome-wise annotation of differentially methylated transcripts and their expression profile in MDD (data presented as log2FC). In the plot, individual tracks or rings represent different annotations. The outermost track displays the cytoband for human reference genome version hg38. The following two tracks display hyper (red) and hypo (blue) methylated transcripts. The outermost periphery of the circos plot represents chromosome number. D MDD-specific percent-wise distribution of m6A sites on coding transcripts is shown in the pie chart. As mapped in the chart, primarily the m6A sites were associated with the coding region (CDS) of mRNA transcript, flanked by 5’ and 3’ untranslated regions (UTRs). E Consensus motifs associated with m6A site enrichment on RNA transcripts. Two motifs were mapped with slight difference at the 3^rd, 4^th, and 8^th nucleotide positions.