Fig. 2: iGCs are required for the behavioral effects of ECS.

A Experimental timeline. Mice received X-irradiation targeting the hippocampus (X-IR) or sham (X-sham). After 8 weeks of recovery, all mice received Cort in drinking water for the duration of the experiment. After 4 weeks, mice received 10 sessions of ECS or Sham (E-sham) and were behaviorally tested in the novelty suppressed feeding test and the forced swim test. B, C Doublecortin (DCX) immunohistochemistry reveals successful ablation of iGCs in X-IR mice vs. X-sham. D On the NSF test, latency to feed is reduced in ECS-treated mice with intact neurogenesis but not in X-IR mice treated with ECS. E On the FST, X-IR mice receiving ECS do not show decreased immobility time when compared to Sham-treated mice. Statistical analysis was performed using two-way ANOVA for normally distributed data (D), and the log-rank Mantel-Cox test to report latency to feed (C); (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).