Fig. 7: Protain associated epigenetic changes.

A Western blots probed for REST, RCAN1, H3C3, PPP1R1B, AQP4 and beta-tubulin. B Quantification of the REST, RCAN1, H3C3, PPP1R1B, AQP4, and acetylated lysine normalized to beta-tubulin from each corresponding biological sample. Data were analyzed using a two-way ANOVA with Sidakâs correction and presented with error bars representing ± SD. *Pâ<â0.05, **Pâ<â0.002, ***Pâ<â0.0002. Each lane represents a biological replicate with male/vehicle (nâ=â5), male/PSI (nâ=â4), female/vehicle (nâ=â3), and female/PSI (nâ=â6). RIPA soluble cerebral cortex, prelimbic cortex and prefrontal cortex. Sex x treatment interaction REST (Fâ=â7.879, pâ=â0.014); RACN1 (Fâ=â5.338, pâ=â0.036); H3C3 (Fâ=â10.29, pâ=â0.0063); AQP4 (Fâ=â5.181, pâ=â0.0391); Acetylated Lysine (Fâ=â5.424, pâ=â0.0354); PPP1R1B (Fâ=â2.716, pâ=â0.1216).