Fig. 1: Expression of GalR1 in the vPFC and the vHC. | Neuropsychopharmacology

Fig. 1: Expression of GalR1 in the vPFC and the vHC.

From: Galanin receptor 1 expressing neurons in hippocampal-prefrontal circuitry modulate goal directed attention and impulse control

Fig. 1

a Representative image of the distribution of GalR1 in the PFC (scale bar: 200 µm). b Quantification of GalR1 mRNA fluorescence intensity in three subdivisions of the PFC (N = 3 animals, 3–4 sections per animal; Cg1: 4.43 ± 1.47, PrL: 4.58 ± 1.91, IL: 6.69 ± 2.79, arbitrary unit: au). Bar chart represents mean ±SEM. Dots represent individual animals. c Magnified image of the distribution of GalR1 in different cortical layers of the IL cortex. d Representative image of the distribution of GalR1 in the vHC (scale bar: 500 µm). e Quantification of GalR1 mRNA fluorescence intensity in the vHC (N = 5 animals, 3–4 sections per animal; DG: 8.73 ± 1.92, CA3: 8.29 ± 1.78, CA2: 8.89 ± 1.58, CA1: 13.76 ± 2.97, arbitrary unit: au). Bar chart represents mean ±SEM. Dots represent individual animals. f Magnified image of the distribution of GalR1 in the different layers of the vCA1/vSub. g–k Co-expression of GalR1 mRNA with glutamatergic neuron marker VGluT1 (GalR1+/VGluT1+ from 642 GalR1+ cells: 75.2 ± 3.2%, GalR1+/VGluT1- from 642 GalR1 cells: 24.6 ± 3.3%; GalR1+/VGluT1+ from 229 VGluT1+ cells: 58.3 ± 5.2%, GalR1−/VGluT1+ from 229 VGluT1+ cells: 41.7 ± 5.2%; 3 animals, 3–4 sections per animal) and the GABAergic neuron marker VGAT (GalR1+/VGAT+ from 647 GalR1+ cells: 8.4 ± 1.0%, GalR1+/VGAT- from 647 GalR1+ cells: 91.6 ± 1.0%; GalR1+/VGAT+ from 83 VGAT+ cells: 66.0 ± 7.4%, GalR1−/VGAT+ from 83 VGAT+ cells: 34.0 ± 7.4%; 2 animals, 2–4 sections per animal) in the IL. Scale bars: 100 µm. l–p Co-expression of GalR1 mRNA with glutamatergic neuron marker VGluT1 (GalR1+/VGluT1+ from 590 GalR1+ cells: 86.0 ± 0.9%, GalR1+/VGluT1- from 590 GalR1+ cell: 14.0 ± 0.9%; GalR1+/VGluT1+ from 340 VGluT1+ cells: 67.8 ± 3.4%, GalR1−/VGluT1+ from 340 VGluT1+ cells: 32.2 ± 3.4%; 3 animals, 2 sections per animal) and the GABAergic neuron marker VGAT (GalR1+/VGAT+ from 610 GalR1+ cells: 8.6 ± 1.3%, GalR1+/VGAT− from 610 GalR1+ cells: 91.4 ± 1.3%; GalR1+/VGAT+ from 74 VGAT+ cells: 66.1 ± 1.3%, GalR1−/VGAT+ from 74 VGAT+ cells: 33.9 ± 1.3%; 1 animal, 3 sections) in the vCA1. Scale bars: 100 µm.

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