Fig. 2: KOR agonists effects on chloroquine phosphate-induced pruritus diuresis, climbing behavior and nesting behavior in male C57BL6/J mice.
A U50,488H, triazole 1.1, and triazole 187 dose-dependently suppress chloroquine-phosphate (CP) (40 mg/kg, s.c. neck) induced scratching in in male C57BLJ/6 mice when administered 10 min before CP. B The number of scratches are shown (n: Veh s.c.: 14; U50 s.c.: 7-12; Tri 1.1 s.c.: 6-12; Veh i.p.: 10 Tri 187 i.p.: 9). C U50,488H, induces more urine production than triazole 1.1 and triazole 187 over a 3 h period compared to vehicle. D The urinary output in µL is shown per dose (n: Veh s.c.: 21; U50: 5-12; Tri 1.1: 6-12; Tri 187: 6-8). E Climbing responses were counted during the urine collection over 3 h. Since U50,488H is sedative at higher doses, the 10 mg/kg dose was used to set the maximum and 15 and 30 mg/kg data were not fit to determine potency in the %MPE curve. F Climbing attempts are shown for each dose (n are: the same as in D). G U50,488H potently disrupts nesting behaviors. H The number of zones cleared are shown for each dose (n: Veh s.c.:9; Veh i.p.: 8; U50: 6; Tri 1.1: 6-7; Tri 187: 5-7). The calculated ED50 from the hyperbolic curves for each compound is presented in Table 1 with 95% CI. Data are presented as mean ± S.E.M. Dose vs. vehicle comparisons were conducted using ordinary one-way ANOVA with Dunnett’s post-hoc test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
