Fig. 3
From: The deubiquitylase USP15 regulates topoisomerase II alpha to maintain genome integrity
USP15 depletion causes anaphase bridges that can lead to micronuclei formation. a, b USP15 depletion in A549 cells significantly increases the number of anaphase chromosome bridges but not the number of lagging chromosomes. a Representative examples of anaphases observed in A549 cells, showing chromosome bridges in USP15 depleted cells. b Examples of each phenotype (top) with quantification of their prevalence below, data show mean of three independent experiments with >50 anaphases counted per condition per experiment (error bars SD, one-way ANOVA with Dunnett’s multiple comparison test *P ≤ 0.05). c Live cell imaging demonstrates that anaphase chromosome bridges (arrow) can resolve into micronuclei (circled) in USP15 depleted cells; the timecourse begins at metaphase and extends beyond cytokinesis. All scale bars are 10 μm
