Table 2 Genes with statistically significant splicing identified by all three bioinformatics methods (FIRMA, MIDAS, ANOSVA) with five and more consecutive exon spliced

From: Alternative transcription of a shorter, non-anti-angiogenic thrombospondin-2 variant in cancer-associated blood vessels

Gene symbol

Number of adjacent exons spliced

FIRMA scores

FIRMAp values

MIDAS scores

MIDASp values

ANOSVA p values

RABGAP1L

5

2.67

0.00009

2.08

0.029123

0.00557443

COL5A2

11

−2.69

0.0001

3.78

0.018022

5.59315E−12

THBS2

8

−2.13

0.00012

−2.82

0.026826

1.82292E−10

CAPRIN1

4

−2.71

0.00016

1.77

0.016125

0.00401313

CCT2

5

−2.91

0.00017

−5.31

0.034639

1.66782E−08

PTK2

4

−2.47

0.00024

1.05

0.029415

0.000841486

CAND1

7

2.94

0.00036

1.72

0.036665

1.18221E−05

ALCAM

6

1.95

0.00036

1.49

0.042686

3.01511E−06

IARS2

6

−2.16

0.00043

4.17

0.02786

0.00132895

GNAS

4

2.09

0.00043

1.26

0.048403

3.58804E−06

CLSPN

12

−1.71

0.00045

−2.78

0.018561

7.88203E−10

COL12A1

5

−2.16

0.00049

3.61

0.017637

1.24805E−11

MANF

8

−1.69

0.00051

4.77

0.028571

1.57539E−08

ESYT1

4

1.78

0.00052

−3.86

0.024699

6.8431E−07

SLC17A5

4

−1.78

0.00054

3.04

0.030499

0.000364402

APP

6

−1.94

0.00064

2.25

0.044619

0.000200504

EZH2

4

4.95

0.00067

3.06

0.046242

2.39594E−10

TPX2

10

−1.54

0.0007

2.58

0.048631

5.44606E−10

CCNA2

4

−1.77

0.00072

2.83

0.032232

1.44783E−10

TRIM33

8

−2.05

0.00073

1.60

0.021483

0.00122048

GDE1

4

−1.61

0.00076

2.90

0.045743

7.47662E−08

RDH10

4

1.95

0.00078

1.91

0.02083

0.000160419

ADAMTS9

10

−1.96

0.00086

−2.23

0.020655

0.000177544

LEMD3

7

2.09

0.0009

2.86

0.048904

0.000502659

BUB1

6

−3.24

0.0009

−2.30

0.020682

0.00287803

TNFRSF21

7

2.05

0.00098

3.26

0.030943

1.01738E−08

MTCH2

5

−1.68

0.00099

−3.38

0.013583

0.00801725

  1. Scores and significance values for genes that have five or more adjacent exons that are spliced; potential alternative transcripts
  2. MIDAS microarray detection of alternative splicing, FIRMA finding isoforms using robust multichip analysis, ANOSVA analysis of splice variation