Fig. 2 | Oncogene

Fig. 2

From: C9orf140, a novel Axin1-interacting protein, mediates the negative feedback loop of Wnt/β-catenin signaling

Fig. 2

Determination of the domains responsible for the interaction between C9orf140 and Axin1. a Identification of the C9orf140 domain responsible for interacting with Axin1. The 293T cells were transfected with Myc-Axin1 and a series of Flag-tagged C9orf140 deletion mutants as depicted in the diagrams. Cell lysates were immunoprecipitated with an anti-Flag antibody, and the precipitates and whole-cell lysates were then analyzed by western blotting. b Identification of the Axin1 domain responsible for interacting with C9orf140. Flag-C9orf140 was co-transfected into 293T cells with Myc-Axin1 or a series of Myc-tagged Axin1 mutants. Flag-C9orf140 was immunoprecipitated, and the precipitates and cell lysates were then subjected to western blot analysis

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