Fig. 2
Trastuzumab-resistant cells are highly aggressive in vivo. a Subcellular localizations of EMT-associated markers E-Cadherin and Vimentin in MKN45, NCI-N87, and MKN45TR, NCI-N87TR cells was assessed through immunofluorescence. b 1 × 105 MKN45 and MKN45TR cells were injected into nude mice through the tail vein for 30 days to evaluate the lungs, livers, and subcutaneous homing potential. Representative figures for liver metastatic tumor (b), HE staining for metastatic liver and lung (c, d). Data for the lung micro invasive foci area, the area of metastatic lung nodules in individual mice was calculated. c HE staining for subcutaneous homing of MKN45 and MKN45TR cells by tail vein injection. d IHC analysis of the expression change of Vimentin and E-Cadherin between MKN45 and MKN45TR group. Representative figures are shown. The data are presented as mean ± SD of three independent experiments
