Fig. 4 | Oncogene

Fig. 4

From: The constitutive activity of the virally encoded chemokine receptor US28 accelerates glioblastoma growth

Fig. 4

US28-targeting nanobodies displace chemokine binding. a Displacement of 125I-CCL5 from US28-expressing HEK293T membranes by periplasmic extracts expressing single nanobody clones. Candidate clones are indicated with blue circles. b, c Displacement of 125I-CCL5 (b) or 125I-CX3CL1 (c) from US28 on HEK239T membranes by monovalent or bivalent nanobodies or unlabeled ligand. Monovalent nanobody: open blue circles, bivalent nanobody: filled red circles and unlabeled ligand: filled black squares. d Lack of displacement of 125I-CX3CL1 from CX3CR1-expressing HEK293T membranes by the US28 nanobodies. The data show mean ± SEM. ***P < 0.001 (unpaired t test)

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