Fig. 5
Nanobodies bind the N-terminus of US28 and detect US28 in GBM tissue. a Binding of monovalent and bivalent US28-nanobodies to US28-expressing HEK239T cells, as detected by flow cytometry. b Nanobody binding to US28 wild type or N-terminally truncated US28-Δ2–22 mutant, as determined by fluorescence microscopy. US28 is detected with rabbit-anti-US28 antibodies (RαUS28), nanobodies with anti-Myc (green) and nuclei with DAPI (blue).c Indication of the binding epitopes of the rabbit-anti-US28 antibodies (C-terminal) and nanobody (N-terminal) on US28. d Immunohistochemistry staining of US28 using bivalent US28-nanobodies. Parallel sections of GBM patient material were stained with hematoxylin (blue) and bivalent US28 nanobody or rabbit-anti-US28 antibodies (brown). IgG isotype control (IgG) was used as control. Nanobodies were detected via their Myc-tag. Scale bars represent 250 μm and 100 μm (inset)
