Fig. 2
From: MASTL overexpression promotes chromosome instability and metastasis in breast cancer
MASTL causes mitotic defects by delaying cell cycle progression. a MCF10A cells transfected with lentivirus coding for MASTL (MASTL) or empty vector controls (Cont.) were analysed by western blot, for MASTL and GAPDH (loading control). MASTL protein overexpression was determined by densitometry (mean ± SEM, n = 3, unpaired Student's t-test, *p < 0.05). b Cell proliferation was assessed using an IncuCyte Zoom over 72 h. Proliferation was measured by %Confluence of the phase contrast channel (mean ± SEM, n = 3, two-way ANOVA with multiple corrections, ***p < 0.001, ****p < 0.0001). c Live-cell microscopy of control and MASTL cells. d Individual cell fate maps for 100+ cells. Average interphase (from first anaphase to second NEBD), and mitotic length (NEBD to anaphase), are shown (mean ± SEM, unpaired Student's t-test, ***p < 0.001). e Mathematical model of mitosis (adapted from Rogers et al. [21]), incorporating twofold increase in MASTL activity to mimic the effects of MASTL overexpression on PP2A-B55 reactivation during mitotic exit
