Fig. 2
ARAF has both allosteric and catalytic activities albeit less than BRAF and CRAF. a As a receiver, ARAF is strongly activated by BRAF, intermediately by CRAF, and weakly by itself through dimerization. The RAF co-activation assays were carried out as before [20, 37]. Briefly, the activator and the receiver were co-expressed in 293T cells, and the phospho-ERK1/2 was measured by immunoblot. b,c As an activator, ARAF stimulates moderately the catalytic activity of ARAF and CRAF receivers, albeit hardly that of BRAF receiver. The RAF co-activation assays were carried out as in a. All images are representative of at least three independent experiments
