Fig. 3

circ-Dnmt1 promoted p53 and Auf1 nuclear translocation. a The circ-Dnmt1- and vector-transfected MCF-7 cells were stained with fluorescence showing expression of Dnmt1 or LC3B. Image J analyses showed that circ-Dnmt1-transfected cells expressed increased levels of Dnmt1 and LC3B (n = 6). b Higher levels of circ-Dnmt1 were significantly detected in nuclei and cytoplasm of the circ-Dnmt1-transfected cells. c Western blotting of tumor lysates showing decreased expression of p53 and increased levels of Dnmt1 in the tumors formed by the circ-Dnmt1-transfected cells. d Protein lysates prepared from circ-Dnmt1- and vector-transfected MCF-7 cells were subject to Western blot. Levels of p53 decreased in the lysate and cytoplasm, but increased in the nuclei of the circ-Dnmt1-transfected cells. Level of Auf1 was not affected by circ-Dnmt1 expression, but decreased in the cytoplasm and increased in the nuclei of the circ-Dnmt1-transfected cells. e Silencing circ-Dnmt1 decreased nuclear translocation of p53 and Auf1