Fig. 4
MYPOP inhibits colony formation of HPV-transformed and non-virally transformed cells. a–c Cells were transfected with either MYPOP expression plasmid or a control plasmid and selected for 6–12 days with G418. Colonies of control or MYPOP-transfected cells were fixed with methanol and stained using crystal violet (upper panel a–c). Plates were quantified using ImageJ plugin “ColonyArea” (lower panel a–c) and values are given as boxplots. a Shown are representative image of SiHa cells and the values obtained from five independent experiments. Control-transfected cells were set to 100%. Data (n = 20) were analyzed using Wilcoxon rank sum test p = 1.451 × 10−11, W = 400. b Shown are representative image of HeLa cells and the values obtained from nine independent experiments. Control-transfected cells were set to 100%. Data (n = 30) were analyzed using Wilcoxon rank sum test p = 2.2 × 10−16, W = 899. c Shown are representative image of HaCaT cells and the values obtained from six independent experiments. Control-transfected cells were set to 100%. Data (n = 22) were analyzed using Welch two-tailed t-test p = 0.00105, t = 3.5361, dF = 39.71; **p ≤ 0.01, ***p ≤ 0.001
