Fig. 6 | Oncogene

Fig. 6

From: MicroRNA 483-3p targets Pard3 to potentiate TGF-β1-induced cell migration, invasion, and epithelial–mesenchymal transition in anaplastic thyroid cancer cells

Fig. 6

MiR-483 targets Pard3. a Putative miR-483 binding sites in the Pard3 3′-UTR. b A luciferase reporter plasmid containing wild-type or mutant Pard3 was co-transfected into HEK293T cells with miR-483 mimics or mimics NC. Luciferase activity was determined at 48 h after transfection using the dual-luciferase assay and was normalized to Renilla luciferase activity. c–f qRT-PCR and western blot assessment of Pard3 expression in 8505C or FRO cells transfected with miR-483 mimics, mimics NC, miR-483 inhibitor, and inhibitor NC. GAPDH was used as a loading control. **p < 0.01, ***p < 0.001, one-way analysis of variance. N = 3 independent experiments with triplicate biological replicates for each line

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