Fig. 1
CXCR4 localizes to the nucleus in RCC, which predicts more metastasis. a Immunohistochemistry analysis of CXCR4 subcellular distribution in primary (n = 11) and metastatic (n = 5) tumors of human RCC. Representative immunohistochemistry images are shown. White scale bar represents 20 μm; blue scale bar represents 5 μm. The proportions of different subcellular localization patterns of CXCR4 were calculated (right). b Immunohistochemistry analysis of CXCR4 subcellular distribution in tissue microarrays. Representative immunohistochemistry images of cytoplasmic, partial nuclear, and total nuclear localization are shown. Cytoplasmic, partial nuclear, and total nuclear localization is defined as <15%, 15–50%, and >50% of cells with CXCR4 nuclear localization, respectively. Black scale bar represents 200 μm; blue scale bar represents 10 μm. c The numbers of different subcellular localization patterns of CXCR4 in the tissue microarrays (p < 0.05) (n = 98). d Kaplan−Meier analysis of metastasis-free survival according to CXCR4 subcellular distribution in primary tumor and adjacent nontumor tissues (p = 0.0284, log-rank test) (n = 98)
