Fig. 2

ZNF185 is upregulated during keratinocyte differentiation and in human epidermis. a RNA expression of ZNF185 in PK and DK HEKn analysed by RNA-seq. b RT-qPCR analysis of the ZNF185 mRNA level in HEKn that had been differentiated for 0, 3, 6, and 9 days. *P < 0.05. Western blot showing the expression levels of ZNF185, p63, and K10 in HEKn differentiated for 0, 3, 6, and 9 days. c Western blot showing the expression levels of ZNF185, p63, and K10 in Ker-CT cells differentiated for 0, 3, 6, 9, 12, and 15 days. d Immunofluorescence analysis of ZNF185 expression in HEKn differentiated for 0, 3, or 6 days. Scale bar: 50 µm. Right panel: 3D rendered confocal imaging of HEKn using alpha-blending algorithm. Scale bar: 20 µm. e Immunofluorescence analysis of ZNF185, K10, Lor, and p63 expression in 3D organotypic skin tissue from Ker-CT cells. Keratin 14 was used as a marker of the basal layer. Scale bar: 50 µm. Upper panel: H&E staining of the 3D tissue. f H&E and IHC for p63 staining of normal human skin. g Immunofluorescence analysis of ZNF185 and p63 expression in human epidermis. Keratin 14 was used as a marker of the basal layer. Scale bar: 100 µm