Fig. 4
ZNF185 silencing causes delayed keratinocyte differentiation. a Heat map of gene expression after ZNF185 silencing by siRNA in differentiated HEKn by microarray analysis. P < 0.05; abs(FC) > 2. b Western blot analysis of ZNF185 and Loricrin levels in differentiated HEKn upon ZNF185 depletion with two different siRNA sequences. c GO terms for downregulated genes from (a). d Heat map of downregulated genes from the “Epidermis Development” group. e qPCR analysis of the mRNA level of several downregulated genes upon ZNF185 depletion in differentiated HEKn with two different siRNA sequences. *P < 0.05, n = 2, **P < 0.05, n = 3. f Immunofluorescence analysis of ZNF185 and E-cadherin levels in human epidermis. Scale bar: 100 µm. Colocalization of ZNF185 and E-cadherin was evaluated using Nikon EZ C.1 software. Pearson’s correlation R = 0.64. g Immunoprecipitation of ZNF185 with anti-HA antibody. Western blot for E-cadherin and HA. h PLA analysis of ZNF185 and E-cadherin interaction in differentiated HEKn. Staining with either anti-ZNF185 alone or anti-E-cadherin alone antibodies was performed as control. Scale bar: 50 µm. Histogram showing one representative experiment. PLA signal counts were normalized by DAPI signal counts. Ten different fields were analysed for each sample
