Fig. 3
CSCC-secreted exosomal miR-221-3p promotes lymphangiogenesis in vitro. a Cellular and exosomal miR-221-3p levels in Ect1 stably transfected with miR-221-3p overexpression (miR-221) or negative control (miR-NC) lentivectors were detected by qRT-PCR. b Cellular and exosomal miR-221-3p levels in Siha stably transfected with miR-221-3p knockdown (anti-221) or negative control (anti-NC) lentivectors were detected by qRT-PCR. c Transwell migration assay in HLECs pre-treated with indicated exosomes. Average migrated cells per field were calculated. d Tube formation assay in HLECs pre-treated with indicated exosomes. Average length of tubes per field were calculated. e Representative micrographs of migration (upper panel) and tube formation assay (lower panel) in HLECs pre-treated with indicated exosomes are shown. Scale bar, upper panel, 50 µm; lower panel, 100 µm. miR-NC exo, Ect1/miR-NC secreted exosomes. miR-221 exo, Ect1/miR-221-3p secreted exosomes. anti-NC exo, Siha/anti-NC secreted exosomes. anti-221 exo, Siha/anti-221-3p secreted exosomes. Error bars represent the mean ± SD of three independent experiments. **, P < 0.01; ***, P < 0.001
