Fig. 2 | Oncogene

Fig. 2

From: The interaction of Lin28A/Rho associated coiled-coil containing protein kinase2 accelerates the malignancy of ovarian cancer

Fig. 2

Lin28A interacts with ROCK2. a ROCK2 was included in the Lin28A-complex through IP combined with mass spectrometry. b Lin28A (Red) and ROCK2 (Green) were co-localized in the cytoplasm peri-nuclear. c IP experiment confirmed the interaction of Lin28A and ROCK2 (but not ROCK1) with anti-Lin28A and anti-ROCK2 (or anti-ROCK1) antibodies. d Domain-based Lin28A mutants were constructed successfully. e The expression of Lin28A mutants was expressed in HEK293 cells examined by western blotting analysis. f Lin28A mutants were transfected into HEK293 cells, respectively, IP with anti-ROCK2 antibody, western blotting with anti-Flag antibody. g Domain-based ROCK2 mutants were constructed successfully. h The expression of ROCK2 mutants was examined by western blotting analysis. i ROCK2 mutants were co-transfected with Flag-Lin28A vector in HEK293 cells, respectively, IP with anti-ROCK2 antibody, Western Blotting with anti-Flag antibody and anti-HA antibody

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