Fig. 2
From: mTOR kinase leads to PTEN-loss-induced cellular senescence by phosphorylating p53
mTORC1 and mTORC2 are activated during PICS. a, b MCF7 cells were transfected with Con Si or PTEN Si for 6 h, and then treated with the indicated inhibitors. After 4 days, SA-β-Gal positivity was assessed (left panel) and the percentage of senescent cells was quantified (right graph) (a). WB analyses were performed 2 days later (b). Rapamycin (Rapa), LY294002 (LY), PP2, PD098059 (PD), SB203580 (SB), and SP600125 (SP) inhibit mTORC1, PI3K, Src, Erk, p38, and JNK, respectively. c WB analyses were performed at the indicated times after siRNA transfection. d MCF cells were transfected with either AKT Si or PTEN Si. After 2 days of transfection, WB analyses were performed. The values represent the mean ± SD; n = 3; *P < 0.05; ***P < 0.001
