Fig. 3
NMH delays the development of leukemia in NOX2-sufficent M-KrasG12D mice. a shows the experimental design of the in vivo experiments. b–c Kras expression in hematopoietic cells (M-KrasG12D) was induced in double transgenic mice (LSL-KrasG12D × Mx1-Cre) by pIpC injections. Mice were treated with NMH (red line) or NaCl (CON; blue line) i.p. thrice weekly for 5 weeks. b Survival of control (blue line) and NMH-treated (red line) mice (n = 28 for NHM, n = 29 for controls; logrank test). c White blood cell counts (WBC) in peripheral blood during disease progression in NMH-treated (red line) and control (blue line) mice (n = 26 for each group, t test). d, e Kras expression was induced in triple transgenic mice (Nox2−/−, LSL-KrasG12D, and Mx1-Cre) denoted Nox2−/− M-KrasG12D mice. Mice were treated with NMH (red line) or NaCl (CON; blue line) i.p. thrice weekly. d Survival of control (blue line) and NMH-treated (red line) triple transgenic mice (n = 8 for controls and n = 7 for the NMH-treated group; logrank test). e Counts of white blood cells in blood during progression of disease of control (blue line) and NMH-treated (red line) mice (n = 8 for controls and n = 7 for NMH-treated mice; t test). *p < 0.05, **p < 0.01, ***p < 0.001
